Figure 5.
ANO5 is not required for exposure of PtdSer or PtdEtn following injury.(A) Representative images showing accumulation of the PtdSer sensor LactC2-Clover after laser-mediated injury. The cell boundary is indicated by a dotted white line. Scale bars = 10 µm. (B) Time course of LactC2-FP accumulation after damage, normalized to initial fluorescence at the injury site. (C) LactC2 AUC for initial (0–100 s) and late (100–437 s) stages of the repair time course. ANO5-KO n = 23 fibers from six mice, WT n = 20 fibers from two mice. (D) PtdEtn, detected by Cy3-conjugated duramycin, appears rapidly at the repair patch following wounding. The cell boundary is marked by a dotted white line. Scale bar = 10 µm. (E) Quantification of PtdEtn kinetics, indicating rapid, ANO5-independent recruitment of PtdEtn to the extracellular surface of muscle fibers after damage. Inset in top left of plot highlights the difference in initial PtdEtn accumulation. (F) Dur-Cy3 AUC for initial (0–100 s) and late (100–437 s) stages of the repair time course. ANO5-KO n = 9 fibers from two mice, WT n = 21 fibers from two mice. Data are mean ± SEM. *, P < 0.05.

ANO5 is not required for exposure of PtdSer or PtdEtn following injury. (A) Representative images showing accumulation of the PtdSer sensor LactC2-Clover after laser-mediated injury. The cell boundary is indicated by a dotted white line. Scale bars = 10 µm. (B) Time course of LactC2-FP accumulation after damage, normalized to initial fluorescence at the injury site. (C) LactC2 AUC for initial (0–100 s) and late (100–437 s) stages of the repair time course. ANO5-KO n = 23 fibers from six mice, WT n = 20 fibers from two mice. (D) PtdEtn, detected by Cy3-conjugated duramycin, appears rapidly at the repair patch following wounding. The cell boundary is marked by a dotted white line. Scale bar = 10 µm. (E) Quantification of PtdEtn kinetics, indicating rapid, ANO5-independent recruitment of PtdEtn to the extracellular surface of muscle fibers after damage. Inset in top left of plot highlights the difference in initial PtdEtn accumulation. (F) Dur-Cy3 AUC for initial (0–100 s) and late (100–437 s) stages of the repair time course. ANO5-KO n = 9 fibers from two mice, WT n = 21 fibers from two mice. Data are mean ± SEM. *, P < 0.05.

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