Architecture of the annexin repair cap is lost in ANO5-KO fibers. (A and B) Representative images of coelectroporated ANXA2 and ANXA5 (A) or ANXA2 and ANXA6 (B) in injured muscle fibers. Scale bars = 10 µm. (C and D) Quantification of the spatial organization of annexin proteins derived from line profiles drawn through the repair cap images taken ∼7 min after injury. ANXA2 and ANXA5: ANO5-KO n = 23 fibers from three mice, WT n = 26 fibers from three mice; ANXA2 and ANXA6: ANO5-KO n = 18 fibers from three mice, WT n = 14 fibers from two mice. (E) Orthogonal views of the annexin repair cap generated from z-stacks acquired ∼10 min after injury. Scale bars = 5 µm. (F) Volumes for individual annexin proteins in repair caps following injury. Z-plane images were acquired every 0.5 µm. ANXA2: ANO5-KO n = 35 fibers from four mice, WT n = 36 fibers from four mice; ANXA5: ANO5-KO n = 32 fibers from three mice, WT n = 37 fibers from four mice; ANXA6: ANO5-KO n = 13 fibers from two mice, WT n = 21 fibers from three mice. Data are mean ± SEM. *, P < 0.05.