Figure S2.
Abnormal annexin trafficking and accumulation in ANO5-KO mouse muscle fibers.(A–D) Representative images of ANXA1 (A), ANXA2 (B), ANXA5 (C), and ANXA6 (D) in WT or ANO5-KO fibers following laser-induced injury. White arrows mark the first appearance of annexin in the cap. Scale bars = 10 µm. ANXA1: ANO5-KO n = 18 fibers, WT n = 14 fibers; ANXA2: ANO5-KO n = 42 fibers, WT n = 37 fibers; ANXA5: ANO5-KO n = 36 fibers, WT n = 29 fibers; ANXA6: ANO5-KO n = 20 fibers, WT n = 31 fibers. (E) Western blot analysis of annexin expression in WT or ANO5-KO mice. Ponceau S is shown as a loading control. (F) Densitometry of annexin Western blots, normalized to Ponceau S and expressed as %WT expression. WT n = 3 mice, ANO5-KO n = 3 mice. (G) Images and quantification of spatial organization of ANXA1 in the repair cap as in Fig. 4, A–D. WT n = 14 fibers pooled from two mice, KO n = 15 fibers pooled from two mice.

Abnormal annexin trafficking and accumulation in ANO5-KO mouse muscle fibers. (A–D) Representative images of ANXA1 (A), ANXA2 (B), ANXA5 (C), and ANXA6 (D) in WT or ANO5-KO fibers following laser-induced injury. White arrows mark the first appearance of annexin in the cap. Scale bars = 10 µm. ANXA1: ANO5-KO n = 18 fibers, WT n = 14 fibers; ANXA2: ANO5-KO n = 42 fibers, WT n = 37 fibers; ANXA5: ANO5-KO n = 36 fibers, WT n = 29 fibers; ANXA6: ANO5-KO n = 20 fibers, WT n = 31 fibers. (E) Western blot analysis of annexin expression in WT or ANO5-KO mice. Ponceau S is shown as a loading control. (F) Densitometry of annexin Western blots, normalized to Ponceau S and expressed as %WT expression. WT n = 3 mice, ANO5-KO n = 3 mice. (G) Images and quantification of spatial organization of ANXA1 in the repair cap as in Fig. 4, A–D. WT n = 14 fibers pooled from two mice, KO n = 15 fibers pooled from two mice.

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