ANO5 accumulates at the plasma membrane in response to wounding. (A and B) Deconvolved images of ANO5 (A) or dysferlin (B) translocation to wound-adjacent plasma membrane in WT or ANO5-KO fibers after injury. Scale bars = 10 µm. (C) Quantification of ANO5 fluorescence at the site of injury in ANO5-KO or WT fibers, normalized to fluorescence before injury. ANO5-KO n = 16 fibers from four mice, WT n = 15 fibers from four mice. (D) Quantification of dysferlin fluorescence in ANO5-KO (red squares) or WT (black circles) myofibers following injury. The time course of hANO5 accumulation in WT fibers is shown as a blue dotted line. ANO5-KO n = 11 fibers from two mice, WT n = 9 fibers from three mice. Data are mean ± SEM.