Figure S5.
Phenotypic analyses of the control and Cep57-, Cep57L1-, and Cep57/Cep57L1-depleted cells in mitosis.(A) Normal bipolar spindle formation (pattern 1) was observed in Cep57L1-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siCep57L1 in the presence of SiR-DNA (200 nM, red). (B) Phenotypic patterns observed in fixed cells are consistent with live-cell imaging analysis. HeLa cells were treated with siControl, siCep57, siCep57L1, or siCep57/Cep57L1 and immunostained with antibodies against centrin (green) and Cep192 (red). (C) Histograms represent the frequency of mitotic cells with the indicated phenotypes observed in B. Values are mean percentages ± SD from three independent experiments (n = 30 for each experiment). (D) Normal bipolar spindle formation observed in control and Cep57L1-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siControl or siCep57L1 in the presence of SiR-DNA (200 nM, red). NEBD indicates nuclear envelope breakdown. (E) Chromosome segregation errors observed in Cep57-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siCep57 in the presence of SiR-DNA (200 nM, red). All scale bars, 5 µm in the low-magnification view, 1 µm in the inset.

Phenotypic analyses of the control and Cep57-, Cep57L1-, and Cep57/Cep57L1-depleted cells in mitosis. (A) Normal bipolar spindle formation (pattern 1) was observed in Cep57L1-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siCep57L1 in the presence of SiR-DNA (200 nM, red). (B) Phenotypic patterns observed in fixed cells are consistent with live-cell imaging analysis. HeLa cells were treated with siControl, siCep57, siCep57L1, or siCep57/Cep57L1 and immunostained with antibodies against centrin (green) and Cep192 (red). (C) Histograms represent the frequency of mitotic cells with the indicated phenotypes observed in B. Values are mean percentages ± SD from three independent experiments (n = 30 for each experiment). (D) Normal bipolar spindle formation observed in control and Cep57L1-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siControl or siCep57L1 in the presence of SiR-DNA (200 nM, red). NEBD indicates nuclear envelope breakdown. (E) Chromosome segregation errors observed in Cep57-depleted cells. HeLa-GFP-centrin1 (green) cells were treated with siCep57 in the presence of SiR-DNA (200 nM, red). All scale bars, 5 µm in the low-magnification view, 1 µm in the inset.

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