Figure 5.
Cep57L1, a paralog of Cep57 conserved in vertebrates, shows a centriolar distribution similar to that of Cep57.(A) Centriolar distribution of Cep57L1 at different cell cycle stages. HeLa cells were immunostained with antibodies against Cep57L1 (red) and centrin (green). Scale bar, 500 nm. OM and NM indicate an old mother centriole and a new mother centriole, respectively. (B) STED images representing top view of Cep57/Cep57L1 (red) and PCNT (green) at mother centrioles. Scale bar, 500 nm. Values are the mean radius ± SD (n = 5). (C) The signals of Cep57L1 at the centrosomes were attenuated by siCep57L1 or siCep57/Cep57L1, but not by siCep57. HeLa cells were treated with siControl, siCep57, siCep57L1, or siCep57/Cep57L1 and immunostained with antibodies against Cep57 (green), Cep57L1 (red), and Cep192 (cyan). Scale bars, 5 µm in the low-magnification view, 1 µm in the inset.

Cep57L1, a paralog of Cep57 conserved in vertebrates, shows a centriolar distribution similar to that of Cep57. (A) Centriolar distribution of Cep57L1 at different cell cycle stages. HeLa cells were immunostained with antibodies against Cep57L1 (red) and centrin (green). Scale bar, 500 nm. OM and NM indicate an old mother centriole and a new mother centriole, respectively. (B) STED images representing top view of Cep57/Cep57L1 (red) and PCNT (green) at mother centrioles. Scale bar, 500 nm. Values are the mean radius ± SD (n = 5). (C) The signals of Cep57L1 at the centrosomes were attenuated by siCep57L1 or siCep57/Cep57L1, but not by siCep57. HeLa cells were treated with siControl, siCep57, siCep57L1, or siCep57/Cep57L1 and immunostained with antibodies against Cep57 (green), Cep57L1 (red), and Cep192 (cyan). Scale bars, 5 µm in the low-magnification view, 1 µm in the inset.

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