Spatial association between microtubules and insulin SGs. (A) 3D rendering of a cell with plasma membrane (transparent gray), microtubules (red), and insulin SGs (orange). Inset shows a magnified region. Scale: cube with a side length of 1 µm. (B) Distribution of the distance of insulin SGs to microtubules (MTs) for one representative low-glucose (blue) and one high-glucose (orange) cell with random distributions ρ_MT represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (C) 3D rendering of a cell with plasma membrane, microtubule-associated SGs (orange), not associated SGs (light gray), and microtubules (red). Scale: cube with a side length of 1 µm. (D) Violin plots depicting the distance of associated and not associated insulin SGs to the plasma membrane (PM). (E) Violin plots depicting the distance of associated and not associated insulin SGs to the nucleus. (F) Violin plots depicting the distance of associated and not associated insulin SGs to the Golgi apparatus. assoc., associated.