Figure 2.
Microtubule network properties and distance distributions. (A) Fully reconstructed microtubule network of one β cell with microtubules in red, centrioles in purple, Golgi apparatus in green, and plasma membrane (PM) in gray transparent. Scale, cube with a side length of 1 µm. (B) Microtubule (MT) length and tortuosity distribution of all seven analyzed cells (blue, low-glucose cells; orange, high-glucose cells). Horizontal and vertical lines signify the mean and the interquartile range, respectively. (C) Rendering of only centriole-connected (centrosomal) microtubules of the same cell as in A. The plots show the distributions of distances of MT ends to centrioles for all analyzed cells. Scale, cube with a side length of 1 µm. (D) Rendering of only Golgi-connected microtubules of the same cell as in A. Scale, cube with a side length of 1 µm. The plots show the distributions of distances of the microtubule ends to Golgi for all analyzed cells. (E) Distribution of the distance of microtubule ends to centrioles for one representative low-glucose (blue) and one high-glucose (orange) cell with random distributions ρCent represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (F) Distribution of the distance of microtubule ends to Golgi membranes for the same cells as in E with random distributions ρGolgi represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (G) Distribution of the distance of microtubule ends to the plasma membrane for the same cells as in E with random distributions ρPM represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (H) Distribution of the distance of microtubule pixels to the plasma membrane for the same cells as in E with random distributions ρPM represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively.

Microtubule network properties and distance distributions. (A) Fully reconstructed microtubule network of one β cell with microtubules in red, centrioles in purple, Golgi apparatus in green, and plasma membrane (PM) in gray transparent. Scale, cube with a side length of 1 µm. (B) Microtubule (MT) length and tortuosity distribution of all seven analyzed cells (blue, low-glucose cells; orange, high-glucose cells). Horizontal and vertical lines signify the mean and the interquartile range, respectively. (C) Rendering of only centriole-connected (centrosomal) microtubules of the same cell as in A. The plots show the distributions of distances of MT ends to centrioles for all analyzed cells. Scale, cube with a side length of 1 µm. (D) Rendering of only Golgi-connected microtubules of the same cell as in A. Scale, cube with a side length of 1 µm. The plots show the distributions of distances of the microtubule ends to Golgi for all analyzed cells. (E) Distribution of the distance of microtubule ends to centrioles for one representative low-glucose (blue) and one high-glucose (orange) cell with random distributions ρCent represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (F) Distribution of the distance of microtubule ends to Golgi membranes for the same cells as in E with random distributions ρGolgi represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (G) Distribution of the distance of microtubule ends to the plasma membrane for the same cells as in E with random distributions ρPM represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively. (H) Distribution of the distance of microtubule pixels to the plasma membrane for the same cells as in E with random distributions ρPM represented by a black line. Red dotted and black dotted lines represent actual and random cumulative distributions, respectively.

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