Expression of GFP:INCENP(FB) rescues the frequency of cells at midbody stage after Chk2 inhibition. (A and B) Localization and mean intensity of phosphorylated Aurora B-Ser331 (pAurora B-Ser331) at the midbody center in BE cells. ***, P < 0.001 (Student’s t test). (C–G and J–L) Localization and mean intensity of GFP, INCENP, and Aurora B proteins at the midbody center in BE cells transfected with siRNA or treated with 10 µM TG003 for 4 h. Values represent mean ± SD from n cells. Values in control were set to 1. (H and I) Frequency of midbody stage BE cells. Values represent mean ± SD from three independent experiments (n > 900). ***, P < 0.001 (ANOVA and Student’s t test). Tubulin values indicate midbody thickness. Insets show 1.6× (A and J) or 3.5× (C–F) magnification of the midbodies. Scale bars, 5 µm.