Chk2 inhibition diminishes localization of CPC proteins to the midbody center in late midbodies. (A and B) Time-lapse microscopy analysis of HeLa cells expressing tubulin:GFP. Cells were untreated (control) or treated with 10 µM Chk2 inhibitor II immediately before filming. Midbodies are shown by solid arrows. Time is from midbody formation to midbody disassembly (dotted arrows). Related to Video 1 and Video 2. (C) Frequency of midbody stage cells. BE cells were transfected as indicated, or treated with 10 µM Chk2 inhibitor II (inhII) for 4 h. Values represent mean ± SD from three independent experiments (n > 900). ***, P < 0.001 (ANOVA and Student’s t test). (D) Midbody cartoon. MTs, microtubules. (E–L) Localization and mean intensity of CPC proteins at the midbody center in BE cells. Individual image planes show 3.5× magnification of the midbodies. (M and N) INCENP localization and mean intensity after treatment of BE cells with 10 µM Chk2 inhII in cytokinesis. Values represent mean ± SD from n cells. Values in control were set to 1. ***, P < 0.001 (Student’s t test). Tubulin values indicate midbody thickness. Insets show 1.6× magnification of the midbodies. Scale bars, 5 µm.