Figure S2.
Related to Fig. 3: Analysis of GFP::MZT-1 localization. (A) Coomassie blue–stained gel showing the purified SPD-5 N-terminal fragments (aa 1–473, SPD-5N) used in the interaction assays. Asterisk marks a SPD-5N breakdown product. (B) Images of in situ–tagged GFP::MZT-1 for the indicated conditions. Red asterisks mark the mutated sites in SPD-5. Times shown in top left of large panels and below smaller panels are in seconds relative to NEBD. Scale bar, 2 µm. (C) Quantification of centrosomal GFP::MZT-1 signal. Values were normalized relative to the mean at NEBD in the presence of WT SPD-5. Error bars are the 95% confidence intervals. n refers to the number of centrosomes. Clust, Cluster; NEBD, nuclear envelope breakdown.

Related to Fig. 3: Analysis of GFP::MZT-1 localization. (A) Coomassie blue–stained gel showing the purified SPD-5 N-terminal fragments (aa 1–473, SPD-5N) used in the interaction assays. Asterisk marks a SPD-5N breakdown product. (B) Images of in situ–tagged GFP::MZT-1 for the indicated conditions. Red asterisks mark the mutated sites in SPD-5. Times shown in top left of large panels and below smaller panels are in seconds relative to NEBD. Scale bar, 2 µm. (C) Quantification of centrosomal GFP::MZT-1 signal. Values were normalized relative to the mean at NEBD in the presence of WT SPD-5. Error bars are the 95% confidence intervals. n refers to the number of centrosomes. Clust, Cluster; NEBD, nuclear envelope breakdown.

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