TFEB screen quality control. (a) For deep sequencing, an average of 1,218 (±305) sorted activated cells per library per biologic repeat were analyzed; number of unique sgRNAs detected per library from a whole-genome screen. sgRNA subpooled library H1 to H7 are color coded. (b) i-vii: sgRNA read counts were quantile normalized following log2-CPM treatment. The coefficient of variation was calculated as described (Robinson et al., 2010). A locally weighted linear regression (lowess) mean curve was fitted to the dispersed data using a smother spanning of 0.2. Black fitted curves designated for mean dispersion of control sample, red fitted curves designated for mean dispersion of control sample; an average number of 2,364,645 (±356,453) reads sequenced from activated sorted cells per subgroup library per biologic repeat.