Experimental and simulated comparison of the efficiency of droplets centering in prophase I versus meiosis I. (A) Radial velocities of droplet centroids as a function of the distance to the oocyte center for droplets centered during prophase I (red squares) and meiosis I (blue squares). Mean and standard deviation are represented. n = 22 oocytes in prophase I and n = 11 oocytes in meiosis I; three independent experiments. (B) Top: Scheme explaining the measure of the centering velocity using an exponential fit on the distance to the oocyte center as a function of time. Bottom: Average of the centering velocity (in μm⋅min−1) for droplets centered during the experiment in prophase I (red histogram) and meiosis I (blue histogram). n = 22 oocytes for prophase I and n = 11 oocytes for meiosis I; error bars show the standard deviation. Three independent experiments. (C) Evolution of the distance of the nucleus centroid to the center of the oocyte as a function of time for 15 different simulations with 200 vesicles per oocyte with a mean radius of 1.4 µm. The average curve appears in dark blue, while all curves are in lighter blue. (D) Measure of radial velocity of the nucleus centroid as a function of time from the 15 different simulations using 200 vesicles per oocyte. The average curve appears in dark blue, while the standard deviation for each point of the average curve appears as lighter blue. (E) Impact of the total number of vesicles per oocyte on the mean centering velocity obtained from simulations with n = 500 vesicles (left, 30 simulations) and n = 200 vesicles (right, 15 simulations). 500 vesicles per oocyte corresponds to prophase I, while 200 vesicles per oocyte corresponds to meiosis I.
