Figure 5.
Oil droplets are also centered in oocytes undergoing meiosis I. (A) Centering of an oil droplet in an oocyte undergoing meiosis I. The images correspond to Video 7. One frame is shown every 140 min. The first frame corresponds to NEBD, a marker of meiosis resumption, and the last one to polar body extrusion (PBE), a marker of meiosis I completion. The initial location of the oil droplet is highlighted by a dotted yellow circle on each picture. Scale bar is 10 µm. (B) An oil droplet observed during meiosis I in an oocyte treated with Cyto D. The images correspond to Video 8. One frame is shown every 140 min. The first frame corresponds to NEBD. Note that oocytes treated with Cyto D do not extrude a polar body. The initial location of the oil droplet is highlighted by a dotted yellow circle on each picture. Scale bar is 10 µm. (C) Trajectories of droplet centroids that are centered during the observation in meiosis I. Time (in minutes) is encoded in color on the centroid trajectories (time is presented as a heat map on the right side of the picture). The coordinates of the centroid droplet are given in the oocyte referential where (0,0) is the center of the oocyte. n = 11 oocytes; three independent experiments. (D) Trajectories of droplet centroids not centrally located at the beginning of the observation in meiosis I oocytes treated with Cyto D. Time (in minutes) is encoded in color on the centroid trajectories (time is presented as a heat map on the right side of the picture). The coordinates of the centroid droplet are given in the oocyte referential where (0,0) is the center of the oocyte. n = 13 oocytes; two independent experiments. (E) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet for oocytes in meiosis I (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 11 oocytes; three independent experiments. (F) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet for oocytes in meiosis I treated with Cyto D (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 13 oocytes; two independent experiments.

Oil droplets are also centered in oocytes undergoing meiosis I. (A) Centering of an oil droplet in an oocyte undergoing meiosis I. The images correspond to Video 7. One frame is shown every 140 min. The first frame corresponds to NEBD, a marker of meiosis resumption, and the last one to polar body extrusion (PBE), a marker of meiosis I completion. The initial location of the oil droplet is highlighted by a dotted yellow circle on each picture. Scale bar is 10 µm. (B) An oil droplet observed during meiosis I in an oocyte treated with Cyto D. The images correspond to Video 8. One frame is shown every 140 min. The first frame corresponds to NEBD. Note that oocytes treated with Cyto D do not extrude a polar body. The initial location of the oil droplet is highlighted by a dotted yellow circle on each picture. Scale bar is 10 µm. (C) Trajectories of droplet centroids that are centered during the observation in meiosis I. Time (in minutes) is encoded in color on the centroid trajectories (time is presented as a heat map on the right side of the picture). The coordinates of the centroid droplet are given in the oocyte referential where (0,0) is the center of the oocyte. n = 11 oocytes; three independent experiments. (D) Trajectories of droplet centroids not centrally located at the beginning of the observation in meiosis I oocytes treated with Cyto D. Time (in minutes) is encoded in color on the centroid trajectories (time is presented as a heat map on the right side of the picture). The coordinates of the centroid droplet are given in the oocyte referential where (0,0) is the center of the oocyte. n = 13 oocytes; two independent experiments. (E) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet for oocytes in meiosis I (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 11 oocytes; three independent experiments. (F) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet for oocytes in meiosis I treated with Cyto D (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 13 oocytes; two independent experiments.

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