Figure 2.
Peripherally injected oil droplets are centered in oocytes in prophase I. (A) Transmitted light images of an oil droplet moving toward the center in a prophase I oocyte (observation at low temporal resolution, Δt = 20 min). Images correspond to Video 3. Frame interval displayed here is every 100 min. Scale bar is 10 µm. The yellow arrow points at the oil droplet. The highly refringent object observed by transmitted light on the first frame of the movie corresponds to the nucleolus inside the nucleus. (B) Trajectories of centroid droplets. The coordinates of the droplet centroid (X,Y) are given in the oocyte referential where (0,0) is the oocyte center. Time (in minutes) is encoded in color on the droplet centroid trajectories (colored bar on the right side of the picture). n = 22 oocytes; three independent experiments. (C) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 22 oocytes; three independent experiments. (D) MSD in micrometers squared of the droplet centroid as a function of the delay (in seconds) for droplets injected in prophase I and observed at high temporal resolution (Δt = 500 ms). n = 29 oocytes in prophase I, and n = 14 oocytes in prophase I + Cyto D. Mean MSD curves are in bold (dark black curve for controls; dark blue curve for Cyto D–treated oocytes). The standard deviations of the MSD curves are presented in gray and light blue quadrants; three independent experiments. (E) Scheme summarizing the fact that the bias toward the center is detected only in the presence of F-actin. The nucleus is in light blue, the nucleolus in dark blue, and the oil droplet in light brown; microfilaments appear in red.

Peripherally injected oil droplets are centered in oocytes in prophase I. (A) Transmitted light images of an oil droplet moving toward the center in a prophase I oocyte (observation at low temporal resolution, Δt = 20 min). Images correspond to Video 3. Frame interval displayed here is every 100 min. Scale bar is 10 µm. The yellow arrow points at the oil droplet. The highly refringent object observed by transmitted light on the first frame of the movie corresponds to the nucleolus inside the nucleus. (B) Trajectories of centroid droplets. The coordinates of the droplet centroid (X,Y) are given in the oocyte referential where (0,0) is the oocyte center. Time (in minutes) is encoded in color on the droplet centroid trajectories (colored bar on the right side of the picture). n = 22 oocytes; three independent experiments. (C) Distance of the droplet centroid to the oocyte center presented as a function of time (in minutes) for each individual droplet (gray curves). The black line represents the distance to the oocyte center as a function of time (in minutes) averaged from the whole dataset of droplet centroid trajectories. n = 22 oocytes; three independent experiments. (D) MSD in micrometers squared of the droplet centroid as a function of the delay (in seconds) for droplets injected in prophase I and observed at high temporal resolution (Δt = 500 ms). n = 29 oocytes in prophase I, and n = 14 oocytes in prophase I + Cyto D. Mean MSD curves are in bold (dark black curve for controls; dark blue curve for Cyto D–treated oocytes). The standard deviations of the MSD curves are presented in gray and light blue quadrants; three independent experiments. (E) Scheme summarizing the fact that the bias toward the center is detected only in the presence of F-actin. The nucleus is in light blue, the nucleolus in dark blue, and the oil droplet in light brown; microfilaments appear in red.

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