Effect on COPI and the Golgi of disrupting ERAS. (A) Distribution of the COPI maker Sec26 before and after anchor-away of the ERAS component Tip20. The strain expressed Sec26-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Tip20-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 30 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate structures was measured for ∼30 individual cells in each sample. Bars indicate SEM. Asterisks indicate statistical significance at ***, P < 0.0001. (C) Distribution of the early Golgi marker Vig4 before and after anchor-away of the ERAS component Tip20. The strain expressed GFP-Vig4 together with the Rpl17-FKBPx4 ribosomal anchor as well as Tip20-FRB. Images were captured before and after treatment with Rapa for 30 min. Scale bar, 1 µm. (D) Quantification of the data from C. The procedure was performed as in B. Asterisks indicate statistical significance at **, P < 0.005