Figure 7.
Effect on ERES of disrupting ERAS. (A) Distribution of the ERES marker Sec31 before and after anchor-away of the ERAS component Tip20. A strain expressed Sec31-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Tip20-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate ERES was measured for ∼20 individual cells in each sample. Bars indicate SEM. (C) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the ERAS component Tip20. A strain expressed Sec31-GFP together with OsTIR1 and Tip20-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (D) Quantification of the data from C. The procedure was performed as in B.

Effect on ERES of disrupting ERAS. (A) Distribution of the ERES marker Sec31 before and after anchor-away of the ERAS component Tip20. A strain expressed Sec31-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Tip20-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate ERES was measured for ∼20 individual cells in each sample. Bars indicate SEM. (C) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the ERAS component Tip20. A strain expressed Sec31-GFP together with OsTIR1 and Tip20-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (D) Quantification of the data from C. The procedure was performed as in B.

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