Figure S6.
Control experiments to verify inactivation of the ERAS component Tip20.(A) Redistribution of FRB-tagged Tip20 by the anchor-away procedure. A strain expressed Tip20-GFP-FRB together with the Rpl17-FKBPx4 ribosomal anchor. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Distribution of the Dsl1 complex subunit Dsl3 before and after auxin-induced degradation of Tip20. A strain expressed Dsl3-GFP together with OsTIR1 and Tip20-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (C) Quantification of the data from B. The percentage of the total GFP signal that was present at punctate ERAS was measured for 30 individual cells in each sample. Bars indicate SEM. Asterisks indicate statistical significance at ***, P < 0.0001.

Control experiments to verify inactivation of the ERAS component Tip20. (A) Redistribution of FRB-tagged Tip20 by the anchor-away procedure. A strain expressed Tip20-GFP-FRB together with the Rpl17-FKBPx4 ribosomal anchor. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Distribution of the Dsl1 complex subunit Dsl3 before and after auxin-induced degradation of Tip20. A strain expressed Dsl3-GFP together with OsTIR1 and Tip20-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (C) Quantification of the data from B. The percentage of the total GFP signal that was present at punctate ERAS was measured for 30 individual cells in each sample. Bars indicate SEM. Asterisks indicate statistical significance at ***, P < 0.0001.

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