Figure 5.
Effect on ERAS and ERES of inactivating COPI. (A) Distribution of the ERAS marker Tip20 before and after anchor-away of the COPI component Sec26. A strain expressed Tip20-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Sec26-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Distribution of the ERAS marker Tip20 before and after auxin-induced degradation of the COPI component Sec26. A strain expressed Tip20-GFP together with OsTIR1 and Sec26-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (C) Quantification of the data from B. The percentage of the total GFP signal that was present at punctate ERAS was measured for ∼20 individual cells in each sample. Bars indicate SEM. ***, the punctate fluorescence in the treated sample was significantly lower at P < 0.0001. (D) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the COPI component Sec26. A strain expressed Sec31-mCherry together with OsTIR1 and Sec26-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (E) Quantification of the data from D. The percentage of the total Sec31-mCherry signal that was present at punctate ERES was measured for ∼30 individual cells in each sample. Bars indicate SEM.

Effect on ERAS and ERES of inactivating COPI. (A) Distribution of the ERAS marker Tip20 before and after anchor-away of the COPI component Sec26. A strain expressed Tip20-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Sec26-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Distribution of the ERAS marker Tip20 before and after auxin-induced degradation of the COPI component Sec26. A strain expressed Tip20-GFP together with OsTIR1 and Sec26-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (C) Quantification of the data from B. The percentage of the total GFP signal that was present at punctate ERAS was measured for ∼20 individual cells in each sample. Bars indicate SEM. ***, the punctate fluorescence in the treated sample was significantly lower at P < 0.0001. (D) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the COPI component Sec26. A strain expressed Sec31-mCherry together with OsTIR1 and Sec26-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (E) Quantification of the data from D. The percentage of the total Sec31-mCherry signal that was present at punctate ERES was measured for ∼30 individual cells in each sample. Bars indicate SEM.

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