Figure S5.
Effect on ERES and ERAS of inactivating Sec23. (A) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the COPII inner layer subunit Sec23. A strain expressed Sec31-GFP together with OsTIR1 and Sec23-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate ERES was measured for ∼30 individual cells in each sample. Bars indicate SEM. Asterisks indicate that the punctate fluorescence in the treated sample was significantly lower at P < 0.0001. (C) Distribution of the ERAS marker Tip20 relative to the general ER marker Sec63 before and after auxin-induced degradation of the COPII inner layer subunit Sec23. A strain expressed Tip20-GFP together with Sec63-mCherry as well as OsTIR1 and Sec23-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm.

Effect on ERES and ERAS of inactivating Sec23. (A) Distribution of the ERES marker Sec31 before and after auxin-induced degradation of the COPII inner layer subunit Sec23. A strain expressed Sec31-GFP together with OsTIR1 and Sec23-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate ERES was measured for ∼30 individual cells in each sample. Bars indicate SEM. Asterisks indicate that the punctate fluorescence in the treated sample was significantly lower at P < 0.0001. (C) Distribution of the ERAS marker Tip20 relative to the general ER marker Sec63 before and after auxin-induced degradation of the COPII inner layer subunit Sec23. A strain expressed Tip20-GFP together with Sec63-mCherry as well as OsTIR1 and Sec23-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm.

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