Effect on ERAS of disrupting ERES. (A) Distribution of the ERAS marker Tip20 before and after anchor-away of the ERES components Sec23 and Shl3. A strain expressed Tip20-GFP together with the Rpl17-FKBPx4 ribosomal anchor as well as Sec23-FRB and Shl23-FRB. Images were captured before and after treatment with rapamycin (Rapa) for 10 min. Scale bar, 1 µm. (B) Quantification of the data from A. The percentage of the total GFP signal that was present at punctate ERAS was measured for ∼30 individual cells in each sample. Bars indicate SEM. ***, the punctate fluorescence in the treated sample was significantly lower at P < 0.0001. (C) Distribution of the ERAS marker Tip20 before and after auxin-induced degradation of the ERES component Sec23. A strain expressed Tip20-GFP together with OsTIR1 and Sec23-AID. Images were captured before and after treatment with IAA for 30 min. Scale bar, 1 µm. (D) Quantification of the data from C. The procedure was performed as in B. ***, P < 0.0001.