Coupled formation of ERES and ERAS tracked using Dsl3. (A) The procedure was as in Fig. S3 A, except that the ERAS marker was Dsl3-mCherry. Shown are frames from Video 4. Scale bar, 1 µm. (B) Quantification of the fluorescence signals from the newly formed structures marked by the arrows in A. Each plot was normalized by setting the highest value to 1. (C) Parallel fusion of ERES structures marked with Sec13 and of ERAS structures marked with Dsl3. The procedure was as in Fig. S3 C, except that the ERAS marker was Dsl3-mCherry. Shown are frames from Video 5. Scale bar, 1 µm.