Figure S3.
Coupled dynamics of ERES and ERAS with swapped fluorescent protein tags. (A) Parallel de novo formation of ERES structures marked with Sec13 and of ERAS structures marked with Tip20. The strain expressed Sec13-GFP together with Tip20-mCherry, using the same marker proteins as in Fig. 3 but swapped fluorescent protein tags. As marked by the arrows, the coupled formation of a new ERES and the associated ERAS were tracked by 4D confocal microscopy. Shown are frames from Video 3. Scale bar, 1 µm. (B) Quantification of the fluorescence signals from the newly formed structures marked by the arrows in A. Each plot was normalized by setting the highest value to 1. (C) Parallel fusion of ERES structures marked with Sec13 and of ERAS structures marked with Tip20. The procedure was as in A, except that the arrows mark two ERES-ERAS pairs that fused to form a single ERES-ERAS pair. Shown are frames from Video 3. Scale bar, 1 µm.

Coupled dynamics of ERES and ERAS with swapped fluorescent protein tags. (A) Parallel de novo formation of ERES structures marked with Sec13 and of ERAS structures marked with Tip20. The strain expressed Sec13-GFP together with Tip20-mCherry, using the same marker proteins as in Fig. 3 but swapped fluorescent protein tags. As marked by the arrows, the coupled formation of a new ERES and the associated ERAS were tracked by 4D confocal microscopy. Shown are frames from Video 3. Scale bar, 1 µm. (B) Quantification of the fluorescence signals from the newly formed structures marked by the arrows in A. Each plot was normalized by setting the highest value to 1. (C) Parallel fusion of ERES structures marked with Sec13 and of ERAS structures marked with Tip20. The procedure was as in A, except that the arrows mark two ERES-ERAS pairs that fused to form a single ERES-ERAS pair. Shown are frames from Video 3. Scale bar, 1 µm.

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