Coupled dynamics of ERAS and ERES. (A) Parallel de novo formation of ERAS structures marked with Tip20 and ERES structures marked with Sec13. A strain expressed Tip20-GFP together with Sec13-DsRed. As marked by the arrows, the coupled formation of a new ERAS and the associated ERES were tracked by 4D confocal microscopy. Shown are frames from Video 1. Scale bar, 1 µm. (B) Quantification of the fluorescence signals from the newly formed structures marked by the arrows in A. Each plot was normalized by setting the highest value to 1. Compared with the Sec13-containing structures, the Tip20-containing structures were more variable and dynamic in shape, so the quantification for Tip20 was noisier. (C) Parallel fusion of ERAS and ERES structures. The procedure was as in A, except that the arrows mark two ERAS-ERES pairs that fused to form a single ERAS-ERES pair. Shown are frames from Video 2. Scale bar, 1 µm.