Localization of Dsl1 complex subunits relative to COPI and Golgi markers. (A) Colocalization of the COPI marker Sec26 with the Dsl1 complex subunit Dsl3. A strain expressed Sec26-GFP together with Dsl3-mCherry. The overlap between Sec26-GFP and Dsl3-mCherry was quantified as in Fig. 1 C. Scale bar, 1 µm. (B) Localization of the COPI marker Sec26 and the ERAS marker Tip20 relative to the late Golgi marker Sec7. Strains expressed either Sec26-GFP or Tip20-GFP together with Sec7-DsRed. Scale bar, 1 µm. (C) Localization of the COPI marker Sec26 and the ERAS marker Tip20 relative to the early Golgi marker Vig4. Strains expressed either Sec26-mCherry or Tip20-mCherry together with GFP-Vig4. Scale bar, 1 µm.