Localization of Dsl1 complex subunits to punctate ER domains in P. pastoris. (A) Localization of the Dsl1 complex subunit Tip20. A strain expressed Tip20-GFP together with the general ER marker DsRed-HDEL. Scale bar, 1 µm. (B) Colocalization of the Dsl1 complex subunits Tip20 and Dsl3. A strain expressed Tip20-GFP and Dsl3-mCherry. Scale bar, 1 µm. (C) Quantification of the data from B. Colocalization of Tip20-GFP and Dsl3-mCherry was measured for each of ∼20 individual cells. Above the horizontal axis, yellow indicates the percentage of the red signal that coincided with green pixels, while below the horizontal axis, yellow indicates the percentage of the green signal that overlapped with red pixels. Bars represent SEM.