DAs are not removed from the M-centriole in mitotic Nek2 KO cells. (A) Representative pictures show Cep164 and ODF2 costaining in RPE1 WT and Nek2 KO cells throughout the cell cycle. γ-tubulin was used as a centrosome marker. DNA was stained with DAPI. The insets in each panel show the enlargement of the centrosome area, and the enlargements on the top or bottom of each panel show each single staining. Scale bars, 20 µm (panel) and 2 µm (inset). (B) Quantification of cell cycle–dependent behavior of indicated DAs in RPE1 WT and Nek2 KO cells (done as described in Fig. 1 D). The quantifications for RPE1 WT cells are identical to those in Fig. 1 D, as experiments were done together. Bar graphs represent mean ± SD. Numbers below the bars represent total number of cells analyzed in three independent experiments. A.U., arbitrary units; n.s., not significant. Significance probability values are: n.s., P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.