Co-localization of the kinase Nek2 with appendages. (A) Representative images show localization of Nek2 and Cep164 in RPE1 cells. γ-tubulin labels centrosomes (indicated by arrows), and DAPI stained the DNA. Scale bar, 20 µm. (B and C) Representative 3D-SIM images of Cep164 and ODF2 costained with Nek2 in RPE1 WT (B) and RPE1 Nek2 KO (C) cells. The association of Nek2 with centrioles is represented in the cartoons. Line graphs show the plot profile of Cep164 or ODF2 (red) and Nek2 (green), plotted according to the line shown in the merge picture. Scale bar, 1 µm. (D) Representative 3D-SIM images of Cep164 and ODF2 costained with Nek2 in RPE1 C-Nap1 KO cells. Scale bar, 1 µm. D, D-centriole; M, M-centriole.