Trpml controls actomyosin contractility and force generation. (A) Sqh-GFP kymographs showing retrograde flow of evaluated conditions (30-min movies, 1-min intervals, 360°/cell). Full videos are included in Video 5. (B) Sqh-GFP retrograde flow speed quantification (n ≥ 3 independent cultures, n ≥ 3 cells/condition). Values are presented as box and whiskers (5%–95%), one-way ANOVA with Tukey's multiple comparisonspost hoc test. Mean values are indicated as “+”; statistically equivalent values are represented with the same letter (P < 0.05). Cg, collagen Gal4 driver. (C) Traction force microscopy (TFM) heat maps of control and trpml1 cells plated on a substrate with 500-Pa stiffness. Videos showing bead displacement are included in Video 6. (D) Quantification of the forces exerted by the hemocytes on average over 15-min movies (n = 3 independent cultures, n ≥ 1 cell/condition). Values are presented as scatter dot plot, indicating mean ± SD t test (*, P < 0.05).
