Figure 3.
Recruitment of Mad1 to unattached KTs and robust SAC signaling require phosphorylation of Megator by Mps1.(A and B) Immunofluorescence images (A) and quantifications (B) of Mad1 at NE of interphase S2 cells depleted of endogenous Megator and expressing Megator-EGFP transgenes (n ≥ 41 cells). (C and D) Immunofluorescence images (C) and corresponding quantification (D) of Mad1 at unattached KTs of S2 cells depleted of endogenous Megator and expressing Megator-EGFP transgenes (n ≥ 125 KTs). (E) Mitotic timings of control and Megator-depleted S2 cells expressing Megator-EGFP transgenes upon addition of taxol (100 nM; n ≥ 11 cells) or colchicine (30 µM; n ≥ 15 cells). In B, data are presented as median with interquartile range; in D and E, data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (Kruskal–Wallis, Dunn’s multiple comparison test). Scale bars, 5 µm (inset, 0.5 µm).

Recruitment of Mad1 to unattached KTs and robust SAC signaling require phosphorylation of Megator by Mps1. (A and B) Immunofluorescence images (A) and quantifications (B) of Mad1 at NE of interphase S2 cells depleted of endogenous Megator and expressing Megator-EGFP transgenes (n ≥ 41 cells). (C and D) Immunofluorescence images (C) and corresponding quantification (D) of Mad1 at unattached KTs of S2 cells depleted of endogenous Megator and expressing Megator-EGFP transgenes (n ≥ 125 KTs). (E) Mitotic timings of control and Megator-depleted S2 cells expressing Megator-EGFP transgenes upon addition of taxol (100 nM; n ≥ 11 cells) or colchicine (30 µM; n ≥ 15 cells). In B, data are presented as median with interquartile range; in D and E, data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (Kruskal–Wallis, Dunn’s multiple comparison test). Scale bars, 5 µm (inset, 0.5 µm).

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