Trpml modulates actomyosin cytoskeleton structure and dynamics. (A) Representative images of larval hemocytes expressing LifeAct-Ruby and sqh-GFP. Shown conditions are control, trpml¹ mutants and RNAi-mediated knockdown, vamp7 RNAi, and double trpml and vamp7 knockdown. Scale bar: 5 µm. (A’) Graphical distribution of LifeAct-Ruby and Sqh-GFP with respect to cell radius. Data are presented as mean ± SEM. A.U., arbitrary units. The dashed line marks the 50% of the maximal signal intensity. (B) Quantification of lamella size with respect to radius (n = 3 independent cultures, n ≥ 3 cells/condition, 30 time points, 360°/cell). Cg, collagen Gal4 driver. (C) Actin dynamics in response to MLSA1 treatment in control and trpml¹ hemocytes. Data are shown as changes in area over time with respect to average (dashed line). Full videos are included in Video 2. (D) Cell dynamics quantified as SD of the area over time (n = 3 independent cultures, n ≥ 4 cells/condition, 30-min movies, 1-min intervals). The values in B and D are presented as box and whiskers, one-way ANOVA with Tukey's multiple comparisons post hoc test. Mean values are indicated as “+”; statistically equivalent values are represented with the same letter (P < 0.05).