Trpml and Vamp7 regulate hemocyte migration through different mechanisms. Representative frames of EGFP-labeled pupal hemocytes migrating in vivo (16 h ± 30 min APF). (A) Control hemocytes display a large lamella that changes its direction and morphology over time. (B)trpml¹ hemocytes present fewer protrusions accompanied by the formation of bleb-like structures, as seen at the rear of the hemocyte labeled with a red asterisk. (C) Hemocyte-specific expression of trpml-myc rescues completely the mutant phenotype. (D)vamp7-deficient hemocytes present large protrusions and do not generate bleb-like structures. Asterisks of the same color indicate the same cell between frames. Scale bar in A–D: 10 µm (n = 3 independent experiments, n ≥ 2 animals/experiment). Full videos are shown in Video 1. (E) Pupal hemocyte migration speed (n ≥ 3 animals/condition, n ≥ 20 hemocytes/animal). Box and whiskers (5%–95%), where mean values are indicated as “+.” One-way ANOVA, with Tukey's multiple comparisons post hoc test. Statistically equivalent values are represented with the same letter (P < 0.05). Hml, hemolectin Gal4 driver.