Lysosomal displacement during phagosome maturation. (A) Representative images of hemocytes before (A) and after (A’) 1 h of culture with fluorescently labeled bacteria. In both A and A', the outlier of the hemocytes is depicted by a white line, and acidic vesicles are labeled with LysoTracker (n = 3 independent cultures, n ≥ 2 hemocytes/condition). Scale bar: 5 µm. (B) Relative dispersion of lysosomes with respect to cell radius before and after 1 h of bacterial exposure. Two-way ANOVA with Sidak's multiple comparisons test (*, P < 0.05). (C) Lysosomal displacement with respect to cell radius. Data are presented as scatter dot plot, indicating mean ± SD; one-way ANOVA with Tukey's multiple comparisons post hoc test. Statistically equivalent values are represented with the same letter (P < 0.05). n.s., not significant. Cg, collagen Gal4 driver.