Apoptotic body degradation is Trpml dependent. LifeAct-Ruby–labeled hemocytes were stained with acridine orange to reveal engulfed apoptotic bodies. (A) Larval hemocytes cultured ex vivo reveal the accumulation of large-sized vesicles stained positively with acridine orange. (B) Quantification of volume occupancy of acridine orange–positive vesicles with respect to cell body volume (n = 3 independent cultures, n ≥ 5 cells/condition). Values are presented as box and whiskers (5%–95%); mean values are indicated as “+. t test (*, P < 0.05).” AO⁺, acridine orange positive vesicles. (C) Pupal hemocytes attached to the basal lamina were imaged after pupae dissection (17-h APF, 29°C) and staining with acridine orange, revealing a similar accumulation of apoptotic bodies in trpml1 mutant hemocytes. Scale bar: 5 µm.