Figure S1. Genetic complementation and... | Rockefeller University Press

Figure S1.

Genetic complementation and rescue of trpml1 phenotypes in phagocytic processing and phagocytosis.(A) Representative images of hemocytes cultured with fluorescently labeled bacteria and LysoTracker probe. Scale bar: 5 µm. (B) Phagocytic processing quantified as the percentage of hemocytes containing bacteria within at least two acidic vesicles after 1 h of culture (n = 3 independent cultures, n ≥ 20 hemocytes/condition). One-way ANOVA with Tukey's multiple comparisons post hoc test; statistically equivalent values are represented with the same letter (P ≤ 0.05). (C) Percentage of hemocytes capable of internalizing bacteria after 15 min of culture (n = 3 independent cultures, n ≥ 40 hemocytes/condition). t test (*, P < 0.05). Cg, collagen Gal4 driver; myc and HA, c-myc and hemagglutinin epitopes. (B and C) Data are presented as scatter dot plot, indicating mean ± SD.

Genetic complementation and rescue of trpml¹ phenotypes in phagocytic processing and phagocytosis. (A) Representative images of hemocytes cultured with fluorescently labeled bacteria and LysoTracker probe. Scale bar: 5 µm. (B) Phagocytic processing quantified as the percentage of hemocytes containing bacteria within at least two acidic vesicles after 1 h of culture (n = 3 independent cultures, n ≥ 20 hemocytes/condition). One-way ANOVA with Tukey's multiple comparisons post hoc test; statistically equivalent values are represented with the same letter (P ≤ 0.05). (C) Percentage of hemocytes capable of internalizing bacteria after 15 min of culture (n = 3 independent cultures, n ≥ 40 hemocytes/condition). t test (*, P < 0.05). Cg, collagen Gal4 driver; myc and HA, c-myc and hemagglutinin epitopes. (B and C) Data are presented as scatter dot plot, indicating mean ± SD.