Shrub/CHMP4B localizes to acentrics as they reintegrate into daughter nuclei. (A) Stills from a video (Video 10) of a mitotic neuroblast expressing I-CreI, H2Av-RFP (magenta), and Shrub-GFP (green). At the time when acentrics began their initial poleward movement, neither acentrics (arrows) nor daughter nuclei were associated with any Shrub-GFP puncta. When acentrics began to rejoin daughter nuclei, Shrub-GFP puncta localized to both acentrics and daughter nuclei (arrowheads), including to the location where the acentric is contacting the daughter nucleus. Time is written in seconds after initial acentric poleward movement. Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (B) Average number of Shrub-GFP puncta that localized to acentrics (purple line) and to nuclei (black line) when acentrics initially segregated poleward (left; n = 15) and when acentrics rejoined daughter nuclei (right; n = 13). For 90 s before and after when acentrics initially segregated poleward, little to no Shrub-GFP puncta localized to acentrics or nuclei. For 90 s before and after acentrics began to contact and reintegrate into nuclei, Shrub-GFP puncta frequently localized to acentrics and to daughter nuclei. Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error.