Micronuclei formation is not correlated to changes in distance of acentrics from nuclei or time of acentric segregation. (A and B) Comparison of the distances of acentrics from daughter nuclei (A; control, n = 15; Comt RNAi, n = 32; Rtnl2 RNAi, n = 23; Shrub RNAi, n = 19) and the times between anaphase onset and acentric segregation (B; control, n = 7; Comt RNAi, n = 12; Rtnl2, n = 7; Shrub RNAi, n = 13). Each dot represents one acentric. Blue dots represent acentrics that rejoin daughter nuclei. Red dots represent acentrics that form micronuclei. For each condition, there is no strong correlation between acentrics that form micronuclei and how far acentrics are from main nuclei at the time of acentric segregation. For each condition, there is no strong correlation between acentrics that form micronuclei and how long after anaphase acentrics began to segregate. Boxes represent interquartile ranges and lines represent medians of the measured data. There were no statistically significant differences between either the distance of acentrics from nuclei (Comt RNAi, P = 0.9; Rtnl2 RNAi, P = 0.7; Shrub RNAi, P = 0.3; two-sided Mann–Whitney–Wilcoxon tests) or the time of acentric segregation (Comt RNAi, P = 0.3; Rtnl2 RNAi, P = 0.07; Shrub RNAi, P = 0.6; independent two-sided t tests) between control and RNAi depleted neuroblasts. See also Fig. 5.