Figure 4.
BAF is enriched on late-segregating acentrics, and membrane between acentrics and daughter nuclei fuses.(A) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP (magenta), and PDI-GFP (Video 5; yellow). Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (A′) Fluorescence intensity of PDI-GFP measured on the nuclei (black line; n = 19) and on the acentrics (purple line; n = 19). Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error. Dashed green lines represent the previously measured average time when acentrics began passing through channels (155 s after acentric segregation). PDI-GFP fluorescence became much stronger on acentrics than on daughter nuclei as cells progressed through anaphase to telophase. See also Fig. S1. a.b.u., arbitrary brightness unit. (B) Stills from videos of mitotic neuroblasts expressing I-CreI, H2Av-RFP, and Lamin-GFP (left), GFP-Nup107 (middle), or PDI-GFP (right). Each image is a single Z-slice of one daughter nucleus. Channels in the nuclear envelope (arrows) formed in all three major components of the nuclear envelope, allowing acentrics to enter daughter nuclei. Scale bars are 2 µm. (C) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP, and PDI-GFP. As the acentric approached the daughter nucleus, nuclear membrane on the acentric and daughter nucleus (arrows) fused (asterisk). Time is written in seconds after initial acentric poleward movement. Each image is a single Z-slice. Scale bar is 2 µm. (D) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP (magenta), and GFP-BAF (green). During telophase, BAF accumulated at the nuclear rim of daughter nuclei and strongly on the late-segregating acentrics (arrows). Time is written in seconds after acentric segregation. Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (D′) GFP-BAF intensity on daughter nuclei (black line; n = 19) and acentrics (purple line; n = 19). Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error. Dashed line represents the average time that acentrics enter nuclear envelope channels (155 s). GFP-BAF is recruited to both daughter nuclei and acentrics as neuroblasts enter telophase. At around the time when acentrics first begin to enter nuclear envelope channels, GFP-BAF is dramatically enriched on acentrics.

BAF is enriched on late-segregating acentrics, and membrane between acentrics and daughter nuclei fuses. (A) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP (magenta), and PDI-GFP (Video 5; yellow). Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (A′) Fluorescence intensity of PDI-GFP measured on the nuclei (black line; n = 19) and on the acentrics (purple line; n = 19). Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error. Dashed green lines represent the previously measured average time when acentrics began passing through channels (155 s after acentric segregation). PDI-GFP fluorescence became much stronger on acentrics than on daughter nuclei as cells progressed through anaphase to telophase. See also Fig. S1. a.b.u., arbitrary brightness unit. (B) Stills from videos of mitotic neuroblasts expressing I-CreI, H2Av-RFP, and Lamin-GFP (left), GFP-Nup107 (middle), or PDI-GFP (right). Each image is a single Z-slice of one daughter nucleus. Channels in the nuclear envelope (arrows) formed in all three major components of the nuclear envelope, allowing acentrics to enter daughter nuclei. Scale bars are 2 µm. (C) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP, and PDI-GFP. As the acentric approached the daughter nucleus, nuclear membrane on the acentric and daughter nucleus (arrows) fused (asterisk). Time is written in seconds after initial acentric poleward movement. Each image is a single Z-slice. Scale bar is 2 µm. (D) Stills from a video of a mitotic neuroblast expressing I-CreI, H2Av-RFP (magenta), and GFP-BAF (green). During telophase, BAF accumulated at the nuclear rim of daughter nuclei and strongly on the late-segregating acentrics (arrows). Time is written in seconds after acentric segregation. Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (D′) GFP-BAF intensity on daughter nuclei (black line; n = 19) and acentrics (purple line; n = 19). Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error. Dashed line represents the average time that acentrics enter nuclear envelope channels (155 s). GFP-BAF is recruited to both daughter nuclei and acentrics as neuroblasts enter telophase. At around the time when acentrics first begin to enter nuclear envelope channels, GFP-BAF is dramatically enriched on acentrics.

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