Late-segregating acentrics are associated with nuclear envelope membrane but not lamina or nuclear pore complexes. (A and B) Stills from videos of mitotic neuroblasts expressing I-CreI and H2Av-RFP (magenta) and either Lamin-GFP (A; Video 3, green) or GFP-Nup107 (B; Video 4, cyan). Daughter nuclei (arrowheads) accumulated Lamin-GFP and GFP-Nup107 during late anaphase/early telophase. However, acentrics (arrows) did not associate with Lamin-GFP and often did not associate with GFP-Nup107. We sometimes observed GFP-Nup107 localize to acentrics near daughter nuclei (Fig. 2 B; 144–234 s). Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions. (A′ and B′) Fluorescence intensity of Lamin-GFP (A′; n = 17) or GFP-Nup107 (B′; n = 19) measured on the nuclei (black lines) and the acentrics (purple lines). Lines represent averages. Dark-shaded regions represent ± the standard error. Light-shaded regions represent ± twice the standard error. Dashed green lines represent the previously measured average time when acentrics begin passing through channels (155 s after acentric segregation). Lamin-GFP and GFP-Nup107 fluorescence intensity increased over time as the nuclear envelope reformed on daughter nuclei but remained consistently low on acentrics. See also Fig. S1. a.b.u., arbitrary brightness unit. (C) Superresolution images of fixed neuroblasts expressing I-CreI. Neuroblasts were stained with DAPI (magenta) and anti-Otefin antibody (green). In anaphase, Otefin did not strongly localize to acentrics (arrows), and nuclear Otefin staining was sporadic. In contrast, during telophase, Otefin localized around the rim of daughter nuclei and strongly on the acentrics. In interphase, Otefin localized around the rim of nuclei. Scale bars are 2 µm. Yellow dashed boxes indicate magnified regions.