Figure S4.
PI4KII and P4M analyses.(A, B, and D) Leica point-scanning confocal images of live late L3 salivary gland cells. (A) Original images without Lightning processing of Fig. 6 A′′ (left), Fig. 6 C′′ (middle), Fig. 6 E′′(right). (B) Brightness-enhanced images of Fig 6, G–G′′ (top row) and Fig 6, H–H′′ (bottom row). (C) Projections of 30 spinning-disc confocal planes of live late L3 salivary gland cells expressing GFP-PI4KII (grayscale) in control (left), Past1110-1 mutant (middle), or Syx16 RNAi (right). Corresponding to Fig. 7, A–C. (D) Brightness-enhanced images of Fig 7, G–G′′ (top row) and Fig 7, H–H′′ (bottom row). B and D were enhanced for brightness to reveal whether 2xP4M localized to immature SGs at a low level.

PI4KII and P4M analyses. (A, B, and D) Leica point-scanning confocal images of live late L3 salivary gland cells. (A) Original images without Lightning processing of Fig. 6 A′′ (left), Fig. 6 C′′ (middle), Fig. 6 E′′(right). (B) Brightness-enhanced images of Fig 6, G–G′′ (top row) and Fig 6, H–H′′ (bottom row). (C) Projections of 30 spinning-disc confocal planes of live late L3 salivary gland cells expressing GFP-PI4KII (grayscale) in control (left), Past1110-1 mutant (middle), or Syx16 RNAi (right). Corresponding to Fig. 7, A–C. (D) Brightness-enhanced images of Fig 7, G–G′′ (top row) and Fig 7, H–H′′ (bottom row). B and D were enhanced for brightness to reveal whether 2xP4M localized to immature SGs at a low level.

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