Figure S3.
PI4KIIΔ characterization.(A–C) Spinning-disc confocal images of live early (A and C) or late (B) L3 salivary gland cells. (A) Early L3 cells expressing GFP-2xFYVE (green) and Sgs3-DsRed (red) in control (top) or PI4KIIΔ mutants (bottom). Colocalization of GFP-2xFYVE and Sgs3-DsRed is marked by yellow arrowheads. Dashed boxes mark regions that are magnified 2.1-fold in the insets. (B) Top: PI4KIIΔ cells expressing CD63-GFP (green) with kinase active mCh-PI4KII (red). Yellow arrowheads mark mCh-PI4KII tubules forming contacts with CD63-GFP–positive SG membranes. Magenta arrowhead marks colocalization of CD63-GFP and mCh-PI4KII on SG membranes. Dashed boxes mark regions that are magnified 1.6-fold in the insets. Bottom: PI4KIIΔ cells expressing CD63-GFP (green) with kinase-dead mCh-PI4KIICAT (red). Yellow arrowheads mark mCh-PI4KIICAT within the lumen of CD63-GFP vesicles. Dashed boxes mark regions magnified 2.1-fold in the insets. (C) Early L3 cells expressing CD63-GFP (green) and Sgs3-DsRed (red) in control (top) or PI4KIIΔ mutants (bottom). Dashed boxes mark regions that are magnified 2.1-fold in the insets.

PI4KIIΔ characterization. (A–C) Spinning-disc confocal images of live early (A and C) or late (B) L3 salivary gland cells. (A) Early L3 cells expressing GFP-2xFYVE (green) and Sgs3-DsRed (red) in control (top) or PI4KIIΔ mutants (bottom). Colocalization of GFP-2xFYVE and Sgs3-DsRed is marked by yellow arrowheads. Dashed boxes mark regions that are magnified 2.1-fold in the insets. (B) Top: PI4KIIΔ cells expressing CD63-GFP (green) with kinase active mCh-PI4KII (red). Yellow arrowheads mark mCh-PI4KII tubules forming contacts with CD63-GFP–positive SG membranes. Magenta arrowhead marks colocalization of CD63-GFP and mCh-PI4KII on SG membranes. Dashed boxes mark regions that are magnified 1.6-fold in the insets. Bottom: PI4KIIΔ cells expressing CD63-GFP (green) with kinase-dead mCh-PI4KIICAT (red). Yellow arrowheads mark mCh-PI4KIICAT within the lumen of CD63-GFP vesicles. Dashed boxes mark regions magnified 2.1-fold in the insets. (C) Early L3 cells expressing CD63-GFP (green) and Sgs3-DsRed (red) in control (top) or PI4KIIΔ mutants (bottom). Dashed boxes mark regions that are magnified 2.1-fold in the insets.

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