Figure 5.
PI4KII localizes to EEs and modulates EE dynamics.(A and B) Nikon point-scanning confocal images of live late L3 salivary gland cells expressing GFP-2xFYVE (green) and Sgs3-DsRed (red) in control (A) or PI4KIIΔ mutant (B). Dashed boxes mark regions magnified 2.8-fold in insets. (C) Plot depicting number of hollow endosomes per cell in control and PI4KIIΔ mutant. Top and bottom bars indicate maxima and minima of the datasets. n = total number of cells in images from six (control) or 10 (PI4KIIΔ) independent salivary glands; ****, P < 0.0001. (D and E) Spinning-disc confocal images of live L3 salivary gland cells expressing GFP-2xFYVE (green) and mCh-PI4KIIATP (red) in control (D) or PI4KIIΔ mutant (E). Dashed boxes mark regions magnified 2.1-fold in insets. (F) Plot depicting number of PI4KII tubules per cell in individual optical sections from control and PI4KIIΔ mutant. Top and bottom bars indicate maxima and minima of the datasets. Black bar indicates the mean. n = total number of cells in images from three independent salivary glands; **, P < 0.01.

PI4KII localizes to EEs and modulates EE dynamics. (A and B) Nikon point-scanning confocal images of live late L3 salivary gland cells expressing GFP-2xFYVE (green) and Sgs3-DsRed (red) in control (A) or PI4KIIΔ mutant (B). Dashed boxes mark regions magnified 2.8-fold in insets. (C) Plot depicting number of hollow endosomes per cell in control and PI4KIIΔ mutant. Top and bottom bars indicate maxima and minima of the datasets. n = total number of cells in images from six (control) or 10 (PI4KIIΔ) independent salivary glands; ****, P < 0.0001. (D and E) Spinning-disc confocal images of live L3 salivary gland cells expressing GFP-2xFYVE (green) and mCh-PI4KIIATP (red) in control (D) or PI4KIIΔ mutant (E). Dashed boxes mark regions magnified 2.1-fold in insets. (F) Plot depicting number of PI4KII tubules per cell in individual optical sections from control and PI4KIIΔ mutant. Top and bottom bars indicate maxima and minima of the datasets. Black bar indicates the mean. n = total number of cells in images from three independent salivary glands; **, P < 0.01.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal