Tsp29Fa is required for normal SG maturation. (A–F) Spinning-disc confocal images of live L3 salivary gland cells expressing Sgs3-DsRed (red; A–C) or Sgs3-GFP (grayscale; D–F). (A–C) Cells from RNAi control (A), Tsp29Fa knockdown (B), or Tsp29Fa knockdown expressing CD63-GFP (green, C). (D–F) Cells from control (D), Tsp29J1a (E), and Df(2L)L7a (F). (G) Box-and-whisker plot (see Materials and methods) showing the distribution of cross-sectional areas of SGs (from A–C). n = total number of SGs in images from three independent salivary glands; ****, P < 0.0001. (H) Box-and-whisker plot (see Materials and methods) showing the distribution of cross-sectional areas of SGs (from D–F). n = total number of SGs in images from three independent salivary glands; ****, P < 0.0001.
