CD63 and the Drosophila homologue Tsp29Fa decorate the limiting membrane of SGs and increase SG size. (A–C, F, G, J, and K) Spinning-disc confocal images of live L3 salivary gland cells. (A–C′′) Cells expressing Sgs3-DsRed (red) with one copy of the AB1-GAL4 driver (A–A′′) or with a single copy of UAS-CD63-GFP (green) driven by a single copy of AB1-GAL4 (B–B′′), or with two copies of UAS-CD63-GFP driven by two copies of AB1-GAL4 (C–C′′). (D) Box-and-whisker plot (see Materials and methods) showing the distribution of cross-sectional areas of SGs (from A–C). n = total number of SGs in images from three independent salivary glands; ****, P < 0.0001. (E) Bar graph showing percentage distribution of SGs with different cross-sectional areas (from A–C). (F and G) Cells expressing Sgs3-DsRed (grayscale) with one copy of AB1-GAL4 (F) or two copies of UAS-Tsp29Fa-Flag-HA driven by one copy of AB1-GAL4 (G). (H) Box-and-whisker plot (see Materials and methods) showing the distribution of cross-sectional areas of SGs (from F and G). n = total number of SGs in images from three independent salivary glands; ****, P < 0.0001. (I) Bar graph showing percentage distribution of SGs with different cross-sectional areas (from F and G).