Figure 10.
Disrupting H3pT3–Survivin interaction increases chromosome missegregation in the absence of H2ApT120-Sgo1 interaction.(A and B) DMSO or BAY 1816032 was added to HeLa cells or the indicated Survivin stable cell lines 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (A). Example images are shown (B). (C and D) DMSO or the indicated inhibitors were added to HeLa cells 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (C). Example images are shown (D). (E and F) DMSO or the indicated inhibitors were added to retinal pigment epithelial-1 cells 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (E). Example images are shown (F). (G and H) DMSO or 5-ITu was added to HeLa or the Sgo1-K492A cells at 9 h after release from STB. Cells were then fixed at 12 h after release for DNA staining. Means and ranges for the percentage of anaphase lagging chromosomes were determined in >200 anaphase cells from two independent experiments (G). Example images are shown (H). (I) DMSO or BAY 1816032 were added to the indicated Survivin stable cell lines 9 h after release from STB, and then MG132 was added 10 h after release. Cells were treated with cold medium 12 h after release and then fixed and immunostained. The collected images were deconvolved and analyzed. (J) Model for the role of H3pT3- and H2ApT120-dependent localization of Aurora B at centromeres in regulating kinetochore function and chromosome segregation. Means and SDs are shown (A, C, E, and G; unpaired t test). N.S., not significant. Arrows point to the lagging chromosome (B, D, F, and H) or the merotelic KT-MT attachment (I). Scale bars, 10 µm.

Disrupting H3pT3–Survivin interaction increases chromosome missegregation in the absence of H2ApT120-Sgo1 interaction. (A and B) DMSO or BAY 1816032 was added to HeLa cells or the indicated Survivin stable cell lines 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (A). Example images are shown (B). (C and D) DMSO or the indicated inhibitors were added to HeLa cells 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (C). Example images are shown (D). (E and F) DMSO or the indicated inhibitors were added to retinal pigment epithelial-1 cells 9 h after release from STB. Cells were then fixed 12 h after release for DNA staining. The percentage of cells with lagging chromosomes was determined in >300 anaphase cells from three independent experiments (E). Example images are shown (F). (G and H) DMSO or 5-ITu was added to HeLa or the Sgo1-K492A cells at 9 h after release from STB. Cells were then fixed at 12 h after release for DNA staining. Means and ranges for the percentage of anaphase lagging chromosomes were determined in >200 anaphase cells from two independent experiments (G). Example images are shown (H). (I) DMSO or BAY 1816032 were added to the indicated Survivin stable cell lines 9 h after release from STB, and then MG132 was added 10 h after release. Cells were treated with cold medium 12 h after release and then fixed and immunostained. The collected images were deconvolved and analyzed. (J) Model for the role of H3pT3- and H2ApT120-dependent localization of Aurora B at centromeres in regulating kinetochore function and chromosome segregation. Means and SDs are shown (A, C, E, and G; unpaired t test). N.S., not significant. Arrows point to the lagging chromosome (B, D, F, and H) or the merotelic KT-MT attachment (I). Scale bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal