Figure 6.
The CHC–GTSE1 interaction is required for stabilization of nonkinetochore MTs. (A) Immunofluorescence images showing STLC-induced monopolar spindles in indicated cell lines and RNAi conditions. Dashed yellow lines indicate cell borders. (B and C) Mean growing MT length per cell (B) and comet number per cell (C) determined following 3D reconstruction from experiment shown in A. (D) PEF assay. Mean kinetochore (CREST) to monopole (PCNT) distance per cell following 3D reconstruction from A. For B–D, n ≥ 37 cells per condition over n = 3 experiments pooled for analysis and presented as box and whisker plots; P values from Wilcoxon test adjusted for multiple comparisons (false discovery rate). n.s., not significant. Numeric data is shown in Table S1.

The CHC–GTSE1 interaction is required for stabilization of nonkinetochore MTs. (A) Immunofluorescence images showing STLC-induced monopolar spindles in indicated cell lines and RNAi conditions. Dashed yellow lines indicate cell borders. (B and C) Mean growing MT length per cell (B) and comet number per cell (C) determined following 3D reconstruction from experiment shown in A. (D) PEF assay. Mean kinetochore (CREST) to monopole (PCNT) distance per cell following 3D reconstruction from A. For B–D, n ≥ 37 cells per condition over n = 3 experiments pooled for analysis and presented as box and whisker plots; P values from Wilcoxon test adjusted for multiple comparisons (false discovery rate). n.s., not significant. Numeric data is shown in Table S1.

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