Sequence alignments of GTSE1 and TACC3. (A) MUSCLE alignment of the N-terminus (amino acids 1–143 from human GTSE1) and C-terminus (amino acids 679–739 from human GTSE1) of potential GTSE1 homologues. Alignment was performed on full-length genes from the indicated organisms. A conserved N-terminal FDFD motif is labeled. Three putative clathrin binding box motifs (C–E) are indicated in red. Note that some nonvertebrates also have putative clathrin binding box motifs in the C-terminal region (red boxes). (B) MUSCLE alignment of the region of TACC3 that interacts with Aurora A and CHC. Alignment was performed on full-length TACC3 homologues from the indicated organisms using MUSCLE. Only the region encompassing the described interaction sites between TACC3 and Aurora A/CHC is presented. Human residues S558, L559, Y560, F563, L566, and L567 (important for CHC interaction) and F525 and L532 (important for Aurora A interaction) are designated by red and black arrows, respectively. These residues appear conserved in the three nonvertebrate species carefully analyzed (sea squirt, sea urchin, and fruit fly).