nop-1, but not centralspindlin, is required for PGC lobe formation. (a–c’) Control, zen-4, and cyk-4 mutant PGCs subjected to temperature-shift regime I. (d–e’) PGCs in control and nop-1 mutants at the indicated time points; t = 0 is just before lobes form in the control. (f–h) Quantification of zen-4 (combined from three experiments), cyk-4 (combined from four experiments), and nop-1 (combined from two experiments) phenotypes. ns, not significant. * indicates nuclear position, and L indicates the lobe. ***, P < 0.0001, Fisher’s exact test. Scale bar, 5 µm.