ect-2 is required for PGC lobe formation. (a and a’) ECT-2-GFP enrichment at the cortex of a PGC (dashed line) in a comma-stage embryo. (b–d’) PGCs in control and ect-2 mutants subjected to temperature-shift regime I. Class I and Class III phenotypes are shown. (e) Quantification of ect-2 mutant PGC lobe formation pooled from four experiments. ***, P < 0.0001, Fisher’s exact test. * indicates nuclear position, and L indicates the lobe. Scale bar, 5 µm.