cyk-1 and nmy-2 are required for PGC lobe formation. (a) Temperature-shift regime I used for experiments shown in b-g. (b–e’) Left: PGCs in embryos of the indicated genotype at the time of upshift. Right: PGCs in embryos of the indicated genotype at time point 1 (t = 108 min). Class I and Class II embryos are shown for nmy-2, with the expanded intercellular bridge indicated in e’ (arrowhead). (f and g) Quantification of cyk-1 (four combined experiments) and nmy-2 (10 combined experiments) mutant PGC lobe formation compared with that of WT embryos on the same microscope slides. (h and i) ZEN-4-YFP^PGC in PGCs before and after lobe formation; yellow dot in schematic indicates a focus of ZEN-4-YFP^PGC. (j) Temperature-shift regime II, used for the experiment shown in k. (k–k’’)nmy-2 mutant embryo subjected to temperature-shift regime II showing an expanded intercellular bridge (arrowhead) marked with ZEN-4-YFP^PGC. ***, P < 0.0001, Fisher’s exact test. * indicates nuclear position, and L indicates the lobe. Scale bar, 5 µm.